Experimental Research on the Cytotoxicity of CdSe Nanocrystals with the Irradiation of Violet Light

Author

Chen, Li ; Zuo, Jing ; Xiao, Hua ; Xiong, Jianwen

Author_Institution

Sch. of Phys. & Optoelectron. Eng., Guangdong Univ. of Technol., Guangzhou, China

fYear

2010

fDate

18-20 June 2010

Firstpage

1

Lastpage

3

Abstract

The present study processed the absorption spectrum of CdSe nanocrystals (Ncs) which was modified by mercaptoacetic acid (MA). Based on this, the LEDs whose emission peak is near the absorption peak of the NCs were selected to form a LED array. The leukemia cell line HL60 was cultured in two 96-well plates and incubated with different concentrations of MA-Coated CdSe NCs. One of the 96-well microplates was irradiated by the LED array mentioned above, and the other wasn´t. Then the viability of the cells on both microplates was determined using CCK-8 assay. The results in vitro demonstrated that the ability of MA-Coated CdSe NCs to induce cell death can be promoted under the irradiation of the light source we used.

Keywords

II-VI semiconductors; biological effects of optical radiation; blood; cadmium compounds; cancer; cellular biophysics; light emitting diodes; nanobiotechnology; nanostructured materials; semiconductor quantum dots; toxicology; CCK-8 assay; CdSe; LED; cell death; cytotoxicity; leukemia cell line HL60; nanocrystals; violet light irradiation; Absorption; Cells (biology); In vitro; Light emitting diodes; Light sources; Medical treatment; Nanocrystals; Nanomaterials; Physics; Quantum dots;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th International Conference on

Conference_Location

Chengdu

ISSN

2151-7614

Print_ISBN

978-1-4244-4712-1

Electronic_ISBN

2151-7614

Type

conf

DOI

10.1109/ICBBE.2010.5516632

Filename

5516632