Rapid Determination of Semi-VOC Using Partly-Immersed Three-Phase Hollow Fiber Liquid Phase Microextraction Combined with High Performance Liquid Chromatography

Author

Hailu Lin ; Hong Yan ; Mingbiao Luo ; Xuejiao Peng ; Wei Liu

Author_Institution

Coll. of Life Sci. & Bio-Eng., Beijing Univ. of Technol., Beijing, China

fYear

2010

fDate

18-20 June 2010

Firstpage

1

Lastpage

4

Abstract

A novel method to determine semi-volatile organic compound(semi-VOC) was established by HPLC coupled with a partly-immersed three-phase hollow fiber liquid phase microextraction (PI-LPME) technique for sample preparation. The influence of extraction parameters including organic solvent, pH of donor and acceptor phase, extraction time, the proportion of hollow fiber in gas and liquid were optimized. Under the optimum conditions, the proposed method provided linear range of 0.05-10 μg/ml with r2 0.9992, detection limit of 5 ng/ml (S/N=3), RSD<;5%(n=5 ) and relative recovery of 95.91-97.95%.

Keywords

biological techniques; chromatography; organic compounds; specimen preparation; high performance liquid chromatography; hollow fiber liquid phase microextraction; organic solvent; pH; partly immersed liquid phase microextraction; sample preparation; semiVOC; semivolatile organic compound; three phase liquid phase microextraction; Active matrix organic light emitting diodes; Chemical analysis; Chemical technology; Chemistry; Coupled mode analysis; Educational institutions; Methanol; Needles; Organic compounds; Solvents;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th International Conference on

Conference_Location

Chengdu

ISSN

2151-7614

Print_ISBN

978-1-4244-4712-1

Electronic_ISBN

2151-7614

Type

conf

DOI

10.1109/ICBBE.2010.5516706

Filename

5516706