Label-Free and Low-Invasive Observations of Living Cells with Stimulated Parametric Emission Microscopy

Author

Itoh, Kazuyoshi

Author_Institution

Dept. of Mater. & Life Sci., Osaka Univ.

fYear

2006

fDate

Oct. 2006

Firstpage

284

Lastpage

285

Abstract

Applications of a novel microscopy technique based on the fourwave mixing (FWM) process that is enhanced by two-photon electronic resonance induced by a pump pulse along with stimulated emission induced by a dump pulse are presented. We demonstrate that our FWM technique can be used to obtain three-dimensional microscopic images of unstained living cells such as a tobacco BY2 cell

Keywords

biological techniques; cellular biophysics; multiwave mixing; optical microscopy; optical parametric devices; stimulated emission; FWM technique; fourwave mixing process; label-free observations; low-invasive observations; microscopy technique; nonlinear optical techniques; pump pulse; stimulated emission; stimulated parametric emission microscopy; three-dimensional microscopic living cell images; tobacco BY2 cell; two-photon electronic resonance; Electron microscopy; Focusing; Nonlinear optics; Optical refraction; Optical scattering; Optical variables control; Photonic crystal fibers; Raman scattering; Stimulated emission; Visualization;

fLanguage

English

Publisher

ieee

Conference_Titel

Lasers and Electro-Optics Society, 2006. LEOS 2006. 19th Annual Meeting of the IEEE

Conference_Location

Montreal, Que.

Print_ISBN

0-7803-9555-7

Electronic_ISBN

0-7803-9555-7

Type

conf

DOI

10.1109/LEOS.2006.279059

Filename

4054168