Title :
Non-viral Charge Reversal Vectors for pDNA Delivery
Author :
Prata, Carla A H ; Li, Yougen ; Luo, Dan ; McIntosh, Thomas J. ; Grinstaff, Mark W.
Author_Institution :
Dept. of Biomed. Eng. & Chem., Boston Univ., MA
fDate :
Aug. 30 2006-Sept. 3 2006
Abstract :
A synthetic vector that transform from a cationic to an anionic lipid intracellularly is described. This charge-reversal lipid was synthesized and characterized, including the supramolecular complex it forms with DNA. Enhanced gene transfection was observed using this synthetic vector compared to current cationic lipids
Keywords :
DNA; bioelectric phenomena; cellular biophysics; genetics; molecular biophysics; anionic lipid; cationic lipid; charge-reversal lipid; cytotoxicity; electrostatic interactions; enhanced gene transfection; nonviral charge reversal vector; pDNA delivery; supramolecular complex; synthetic vector; Biomedical engineering; Chemistry; Cities and towns; DNA; Degradation; Design for experiments; Engineering in medicine and biology; Lipidomics; Temperature; USA Councils;
Conference_Titel :
Engineering in Medicine and Biology Society, 2006. EMBS '06. 28th Annual International Conference of the IEEE
Conference_Location :
New York, NY
Print_ISBN :
1-4244-0032-5
Electronic_ISBN :
1557-170X
DOI :
10.1109/IEMBS.2006.260181
