Fabrication of impedimetric sensors for label-free Point-of-Care immunoassay cardiac marker systems, with passive microfluidic delivery

Miniaturised point-of-care cardiac marker sensors are being developed, based on impedimetric sensing of cardiac enzyme capture by an antibody layer immobilised on a planar gold electrode sensor. Gold/Ti-on-glass substrates have been used, in a 2 electrode configuration, with antibodies immobilised on the working electrode. Microfluidic structures have been fabricated by a CO₂ laser, in 25 mum thick pressure sensitive adhesive (PSA), on a PMMA lid, and the structure bonded on top of the planar sensor. Microfluidic blood/serum delivery has been investigated using a visualisation dye. Some flow problems are observed if the sensor is exposed to air for several days, suggesting that flow channel nanopillars and hermetic encapsulation may be required to guarantee flow properties in commercially produced modules. Work is ongoing to characterise the impedimetric signal changes for myoglobin capture by antimyoglobin, using these sensors. Fifty micron thick PSA, incorporating a robust spacer layer, will be used to give better definition of channel walls