Temperature Control in Mini-Bioreactor and Its Impact to Immunoassay

In this paper, the precise temperature control incorporated in the mini-bioreactor for biochemical reaction was developed. The temperature control system can be operated from computer. The variation of temperature is within ± 0.2°C using fuzzy control algorithm. Compared with conventional oven, it has 10-fold precision. It also has better heating efficiency with rapid rising time to reach the desired temperature. The experimental results of immunoassays show that the temperature control has a great impact to immnuoreaction. Under a stable temperature environment, the variation of fluorescence intensities scanned after immunoassays is smaller for all titration concentrations. The temperature control system in mini-bioreactor can promote better experimental results of immunoassay.