Handling of single DNA molecule by controlling local temperature

Author

Katsura, Shinji ; Ishii, Ryuji ; Nishioka, Masateru ; Sakaguchi, Yasuo ; Matsuzawa, Yukiko ; Mizuno, Akira

Author_Institution

Dept. of Ecological Eng., Toyohashi Univ. of Technol., Japan

Volume

4

fYear

1996

fDate

6-10 Oct 1996

Firstpage

1927

Abstract

Laser tweezers are useful tools for manipulation of sub-micrometer particles, but single DNA molecules cannot be manipulated. A novel manipulation method for a single DNA molecule has been developed. A sample solution was frozen and a DNA molecule was embedded in the solid phase of solution. Irradiating by a focused YAG laser, the local area of the frozen solution can be melted. The molten area can be moved and a single DNA molecule can be transported. Further, this technique is useful for regulating the chemical reaction in the local area. The enzymatic reaction of a DNA molecule in the local region was experimentally confirmed in this research

Keywords

DNA; biocontrol; biological techniques; biotechnology; freezing; laser beam applications; melting; molecular biophysics; YAG; YAl5O12; chemical reaction; enzymatic reaction; focused YAG laser irradiation; freezing; laser tweezers; local area; local temperature control; manipulation; molten area; sample solution; single DNA molecule handling; solid solution phase; Biomedical optical imaging; Charge carrier processes; Charge-coupled image sensors; Chemical lasers; DNA; Glass; Laser theory; Microscopy; Power lasers; Temperature control;

fLanguage

English

Publisher

ieee

Conference_Titel

Industry Applications Conference, 1996. Thirty-First IAS Annual Meeting, IAS '96., Conference Record of the 1996 IEEE

Conference_Location

San Diego, CA

ISSN

0197-2618

Print_ISBN

0-7803-3544-9

Type

conf

DOI

10.1109/IAS.1996.563830

Filename

563830