Survivin of Antisense Oligodeoxynucleotides Reverse Apoptotic Resistance Induced by Cisplatin in Lung Cells

We explore whether antisense oligodeoxy-nucleotides (ASODN) targeting survivin gene can reverse apoptotic resistance induced by cisplatin in human lung adenocarcinoma cells. A549 / CDDP cell lines were cultured routinely with RPMI-1640 medium. Survivin ASODN mediated by cytofectin was transfected into the A549 / CDDP cells. The influence of ASODN transfection on apoptosis was determined by Diphenylamine assay (DPA) , caspase-3 colorimetric assay and flow cytometry . 3(4 , 5-dimethyl-thiazol-2-yl)-2 , 5-diphenyl tetrazolium bromide(MTT)assay was performed to detect the cel viability , half-maximum inhibitory concentration OC,o)and cisplatin resistant index were thereby calculated, respectively. In the A549 / CDDP cells transfected by survivin ASODN for 48 hrs, the percentage of DNA fragmentation, apoptotic index and caspase-3 activity was enhanced to(43.55± 6.07)%, (34. 03±2. 25 and (1. 1298+0. 2502), respectively (p<;0.05), it was obviously significant, compared to the controls. While combination with ASODN and 10 bmol / L cisplatin caused far more obvious apoptotic alterations in the cels, of which the percentage of DNA fragmentation, AI and caspase-3 activity was reached to (76.73+2.04)%, (65.85+ 5.47)% and (1.6805+0.2758) (p<;0.05 ), respectively, and even compared to single ASODN group, it was still significant (p<;0.05). Transfected with survivin ASODN single and with combination of cisplatin for 48 hrs, the rate of cel growth inhibition in the resistant cels was enhanced to 59.3 % and 83.7 %, respectively (p <;0.05), while cell viability inversely had the lowest reduction. And the half-maximum inhibitory concentration of cisplatin was reduced from(225.03± 10.59) μmol / L to (158.84+ 4.256)μmol / L, while the resistant index was conversely reduced from 11.9 to 8.39. Non-sense oligodeoxynucleotides (NSODN1 and liposome had no effect on the cells(p>0.05). Transfection of antisense oligodeoxynucleotides t- - argeting survivin gene can reduce the apoptosis threshold, thereafter to great extent reverse apoptotic resistance induced by cisplatin in cisplatin-resistant human lung adenocarcinoma in vitro.