Title :
The Purification of Alkaline and Thermostable Lipase from a Newly Isolated Strain Acinetobacter calcoaceticus 1-7
Author :
Wang, HaiKuan ; Ma, HuiJing ; Zhong, ShaoJiong ; Wei, YuJie ; Qi, Wei
Author_Institution :
Key Lab. of Ind. Microbiol., Tianjin Univ. of Sci. & Technol., Tianjin, China
Abstract :
A newly isolated moderate thermophilic strain 1-7 producing alkaline and thermostable lipase previously was identified as Acinetobacter calcoaceticus, which was isolated from the soil collected from bay of Bohai in China. The lipase showed maximum activity at pH 9.0 and high thermostability. The lipase from Acinetobacter calcoaceticus 1-7 was purified by ammonium sulfate precipitation, ion exchange chromatography and Sephadex G-75 chromatography. The molecular mass (Mr) of the lipase was estimated to be 30 kDa using SDS-PAGE.
Keywords :
biochemistry; biological techniques; biotechnology; chromatography; enzymes; microorganisms; precipitation (physical chemistry); proteomics; purification; thermal stability; Acinetobacter calcoaceticus 1-7; Bohai; China; Sephadex G-75 chromatography; alkaline lipase; ammonium sulfate precipitation; ion exchange chromatography; isolated moderate thermophilic strain 1-7; molecular mass; purification; thermostable lipase; Biochemistry; Capacitive sensors; Chemical industry; Conducting materials; Microorganisms; Petroleum; Purification; Soil; Temperature; Water;
Conference_Titel :
Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th International Conference on
Conference_Location :
Chengdu
Print_ISBN :
978-1-4244-4712-1
Electronic_ISBN :
2151-7614
DOI :
10.1109/ICBBE.2010.5517247
