Title :
Cloning of Upstream Region of cyp51 Gene from Ustilago maydis and Analysis by Bioinformatics
Author :
Li, Chen ; Li, Shuxiang ; Yuan, Liling ; Liu, Yi ; Yang, Xueting ; Yuan, Yongze ; Liu, Deli ; Yang, Jiangke ; Yan, Yunjun
Author_Institution :
Hubei Key Lab. of Genetic Regul. & Integrative Biol., Central China Normal Univ., Wuhan, China
Abstract :
CYP51 of Fungi, known as sterol 14-α-demethylase (P450 14DM) is a key enzyme in the biosynthesis of membrane ergosterol. It will lead to the destruction and loss of function of membrane structure and ultimately to fungal death without CYP51. Based on the 5´-end nucleotide sequence of cyp51, the upstream region of cyp51 was obtained by chromosome walking technique to investigate the regulatory mechanism of cyp51 gene expression. The upstream fragment of 490bp was successfully cloned and sequenced. Analyzed by bioinformatics software, the transcription initiation site of cyp51 was predicted by the NNPP software is located at 134bp in front of ATG, while several cis-acting elements are also analyzed by TFSEARCH 1.3 software. The current study was benefit to further investigating the regulation of cyp51 over-expression and molecular mechanism involved in fungicides resistance of Ustilago maydis in future.
Keywords :
bioinformatics; biomembranes; cellular biophysics; enzymes; genetics; microorganisms; molecular biophysics; molecular configurations; 5´-end nucleotide sequence; Fungi; NNPP software; Ustilago maydis; bioinformatics; biosynthesis; chromosome walking technique; cyp51 gene cloning; cyp51 over-expression; enzyme; fungal death; membrane ergosterol; membrane structure; sterol 14-α-demethylase (P450 14DM); Bioinformatics; Biological cells; Biomembranes; Cloning; DNA; Fungi; Genomics; Immune system; Legged locomotion; Sequences;
Conference_Titel :
Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th International Conference on
Conference_Location :
Chengdu
Print_ISBN :
978-1-4244-4712-1
Electronic_ISBN :
2151-7614
DOI :
10.1109/ICBBE.2010.5517346
