Title :
Synthesis and Identification of Artificial Antigen for Bensulfuron-Methyl
Author :
Yu Xiao-Wei ; Wei Chen-xi ; Xia Ying ; Zhang Ya-ran ; Yuan Jun-lin
Author_Institution :
Hubei Key Lab. of Genetic Regul. & Integrative Biol., Huazhong Normal Univ., Wuhan, China
Abstract :
In this study, artificial antigens for Bensulfuron-methyl (BSM) were prepared by means of two-step procedure of glutaraldehyde method. The hapten BSM was covalently attached to ovalbumin to synthesis immunogen (BSM-OVA), and hapten Benzyl sulfonamide (BS) was linked to BSA to prepare coating antigen (BS-BSA). The conjugates were identified by UV spectrophotometry and SDS-PAGE. After immunized with BSM-OVA to BALB/c mice, high titer and sensitive polyclonal antibodies against bensulfuron-methyl herbicide were obtained. The titer was 1:1.28 × 105. The IC50 was 0.032 μg/mL. The results indicated that the artificial antigen for BSM synthesized successfully, and it could be used to produce monoclonal antibodies for BSM.
Keywords :
agrochemicals; biotechnology; molecular biophysics; proteins; spectrophotometry; UV spectrophotometry; artificial antigens; bensulfuron-methyl; bensulfuron-methyl herbicide; coating antigen; glutaraldehyde method; hapten benzyl sulfonamide; immunogen; monoclonal antibodies; ovalbumin; polyclonal antibodies; Agriculture; Animals; Biology; Coatings; Genetics; Humans; Immune system; Mice; Organisms; Water pollution;
Conference_Titel :
Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th International Conference on
Conference_Location :
Chengdu
Print_ISBN :
978-1-4244-4712-1
Electronic_ISBN :
2151-7614
DOI :
10.1109/ICBBE.2010.5517356
