The mechanism of β-cyclodextrin on the 11β-hydroxylation biotransformation of steroid

Author

Shen, Yanbing ; Ma, Bing ; Zheng, Yu ; Luo, Jianmei ; Zhi, Yongrun ; Wang, Min

Author_Institution

Key Lab. of Ind. Microbiol., Minist. of Educ., Tianjin, China

Volume

5

fYear

2010

fDate

16-18 Oct. 2010

Firstpage

1964

Lastpage

1967

Abstract

In this paper, the biotransformation of 17α-hydroxypregna-4-ene-3 20-dione-21-acetate (RSA) and 17α-hydroxypregna-4-ene-3 20-dione (RS) by Absidia coerulea AC307 was selected as a model system for evaluating the mechanism of β-cyclodextrin (β-CD) on the the steroid 11β-hydroxylation biotransformation process. It was found that the addition of cyclodextrin to RS and RSA biotransformation systems could effectively increase the speed of biotransformation and decrease the reactant´s absorption by microorganism. There may be some relation between the two kinds affection induced by cyclodextrin, and the idea can be validated by Shewmon P G´s theory. It showed that cyclodextrin can affect the steroid-permeating capability of the microorganism, and that seemed to be favourable for reactant entered into the microorganism.

Keywords

biochemistry; cellular biophysics; enzymes; microorganisms; molecular biophysics; β-cyclodextrin; 11β-hydroxylation biotransformation; 17α-hydroxypregna-4-ene-3,20-dione-21-acetate; Absidia coerulea AC307; Shewmon P G´s theory; microorganism; reactant absorption; steroid-permeating capability; Absorption; Biochemistry; Compounds; Microorganisms; Oxidation; Production; Substrates; 11β-hydroxylation; Cyclodextrin; absorption; biotransformation; steroid;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Engineering and Informatics (BMEI), 2010 3rd International Conference on

Conference_Location

Yantai

Print_ISBN

978-1-4244-6495-1

Type

conf

DOI

10.1109/BMEI.2010.5639462

Filename

5639462