DNA quantification with an electrochemiluminescence microcell

Author

Hsueh, Yun-Tai ; Collins, Scott D. ; Smith, Rosemary L.

Author_Institution

Dept. of Electr. Eng. & Comput. Sci., California Univ., Davis, CA, USA

Volume

1

fYear

1997

fDate

16-19 Jun 1997

Firstpage

175

Abstract

Describes the detection and quantification of DNA strands using an electrochemiluminescence (ECL) instrument with microfabricated excitation/detection cell. The microcell contains thin film Pt electrodes, fluid microchannels and a silicon PIN diode photodetector. The DNA strands are labeled with an electrochemically exited, luminescing molecule: tris( 2, 2´ bipyridyl) ruthenium (TBR). In order to separate the free label from labeled DNA, the DNA strands are attached to paramagnetic beads with a biotin-streptavidin linkage. With a 1 cm² Pt anode, a detection limit of 40 femtomoles of DNA is obtained with a silicon PIN diode photodetector operating at room temperature. The overall size of the microcell is only 1.5×2×0.3 cm with a sample volume of 150 μL

Keywords

DNA; biological techniques; chemiluminescence; electrochemistry; microelectrodes; molecular biophysics; p-i-n photodiodes; photodetectors; 0.3 cm; 1 cm; 1.5 cm; 2 cm; DNA quantification; DNA strands; Pt; Si; Si PIN diode photodetector; biotin-streptavidin linkage; detection; detection limit; electrochemically exited luminescing molecule; electrochemiluminescence instrument; electrochemiluminescence microcell; fluid microchannels; free label; labeled DNA; microcell size; microfabricated excitation/detection cell; paramagnetic beads; room temperature; sample volume; thin film Pt electrodes; tris( 2, 2´ bipyridyl) ruthenium; Couplings; DNA; Electrodes; Instruments; Microcell networks; Microchannel; Paramagnetic materials; Photodetectors; Semiconductor thin films; Silicon;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid State Sensors and Actuators, 1997. TRANSDUCERS '97 Chicago., 1997 International Conference on

Conference_Location

Chicago, IL

Print_ISBN

0-7803-3829-4

Type

conf

DOI

10.1109/SENSOR.1997.613611

Filename

613611