Degradation of Hemicelluloses of Cottonseed Coat by Xylanase

The hemicelluloses were separated from the cottonseed coat and degraded with Pulpzyme HC. The digestion degree value was calculated by the equation depended on the release of reducing sugars concentration which was determined from DNS fitting curve equation. The optimum enzyme reaction temperature was 60°C and pH was 5.5. The duration of 80 min was chose as the most suitable enzymolysis time. The degradation degree value was 57.5% when 0.24 ml pure Pulpzyme HC was added with one milligramme hemicelluloses powder. The K⁺ content substrate (0.5 mmol L^-1) enhanced the enzymic degradation of hemicelluloses. The other metal ions were found to be some inhibitors of Pulpzyme HC. The effects of chelating agent (EDTA) were complex and no trend was observed.