Effects of Chloroform Extracts from Kochia scoparia on Protect Enzyme activity of Tetranychus viennensis

There were few reports mentioned changes of protective enzyme activities of Tetranychus viennensis after exposed to pesticides by now. Our previous study had showed that the chloroform extracts of Kochia scoparia had effective acaricidal activity against T. viennensis. Here, the toxicosis symptoms of T. viennensis were detaiedly studied after exposed to the chloroform extracts of K. scoparia KFCE-2. We found that T. viennensis exposed to extract KFCE-2 showed typical toxicosis symptoms including over-excitation, uncoordinatedly moving, turning in circles, limb twitching and egg production decreasing in the contact action bioassay. Afterwards, we measured the potential changes of three protective enzymes [peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT)] of T. viennensis in response to extract KFCE-2. Treatment with extract KFCE-2 enhanced SOD activities during the 24 hr after treatment, while the POD and CAT activity were also enhanced except two periods: the 7^th - 11^th hr (7^th - 14^th hr for CAT activity) and the 20^th -24^th hr after treatment. During these two periods, POD and CAT activity were inhibited. The change of the protective enzyme activities may affect the removal of the toxic oxygen free radical species, which may be one of acaricidal mechanisms of K. scoparia extract KFCE-2 against T. viennensis.