Quantitative Evaluation of Micro-motion of Vascular Endothelial Cells in Electrical Cell-substrate Impedance Sensing (ECIS) Method Using a Precision Mathematical Model

Author

Goda, Noriko ; Yamamoto, Yoshitake ; Nakamura, Takao ; Kusuhara, T. ; Mohri, Satoshi ; Kataoka, Noriyuki ; Kajiya, Fumihiko

Author_Institution

Okayama Univ. Med. Sch.

fYear

2006

fDate

9-12 May 2006

Firstpage

23

Lastpage

26

Abstract

We constructed a precision mathematical model for quantitative evaluation of the micro-dynamics of cultured cells measured with ECIS (electrical cell-substrate impedance sensing) system that could separately estimate cell-to-cell distance and cell-to-substrate distance. For wide applications of this method, we constructed mathematical models describing for some kinds of cell and some kinds of confluent conditions. The mathematical models of these impedances could be fit well especially from 1 kHz to 10 kHz, which were most interesting frequency range for micro-dynamic analysis in ECIS methods

Keywords

biological techniques; cellular biophysics; physiological models; 1 to 10 kHz; ECIS; cell culture; cell-to-cell distance; cell-to-substrate distance; electrical cell-substrate impedance sensing method; microdynamic analysis; micromotion; precision mathematical model; quantitative evaluation; vascular endothelial cell; Capacitance; Cells (biology); Electrodes; Equivalent circuits; Frequency; Gold; Immune system; Impedance; Mathematical model; Power system modeling; Cell culture; ECIS; Mathematical model;

fLanguage

English

Publisher

ieee

Conference_Titel

Microtechnologies in Medicine and Biology, 2006 International Conference on

Conference_Location

Okinawa

Print_ISBN

1-4244-0338-3

Electronic_ISBN

1-4244-0338-3

Type

conf

DOI

10.1109/MMB.2006.251480

Filename

4281298