Development and Evaluation of the Integrated In Situ Analyzer for Gene-"IISA-Gene" for Microbiology in Extreme Environments

Author

Fukuba, Tatsuhiro ; Fujii, Teruo

Author_Institution

Inst. of Ind. Sci., Tokyo Univ.

fYear

2006

fDate

9-12 May 2006

Firstpage

184

Lastpage

187

Abstract

In this study an integrated in situ analyzer system for gene analysis (IISA-Gene) is developed to perform gene analysis of microorganisms inhabiting in extreme environments such as deep-sea or deep subsurface. Microfabricated chips made of PDMS and glass is employed to achieve a totally miniaturized and integrated system. Bacterial cell lysis, DNA purification, PCR amplification and amplified fragment detection can be performed with IISA-Gene system including miniaturized pumping and optical detection device. The IISA-Gene developed in this study was evaluated and operated in real deep-sea environment

Keywords

DNA; bioMEMS; biosensors; cellular biophysics; microorganisms; microsensors; oceanographic techniques; optical sensors; seawater; DNA purification; IISA-Gene system; PCR amplification; PDMS substrate; bacterial cell lysis; chip microfabrication; deep subsurface; deep-sea; fragment detection; gene analysis; glass substrate; integrated in situ analyzer; microbiology; optical detection device; DNA; Glass; Microorganisms; Optical detectors; Optical devices; Optical pumping; Performance analysis; Purification; Stimulated emission; Ultraviolet sources; DNA purfication; Gene analysis; IISA-Gene; PCR; in-situ analysis;

fLanguage

English

Publisher

ieee

Conference_Titel

Microtechnologies in Medicine and Biology, 2006 International Conference on

Conference_Location

Okinawa

Print_ISBN

1-4244-0338-3

Electronic_ISBN

1-4244-0338-3

Type

conf

DOI

10.1109/MMB.2006.251523

Filename

4281341