Development of in situ ATP quantitative analysis system "IISA-ATP"

Author

Fukuzawa, Noriyuki ; Fukuba, Tatsuhiro ; Fujii, Teruo

Author_Institution

Inst. of Ind. Sci., Tokyo Univ.

fYear

2006

fDate

9-12 May 2006

Firstpage

260

Lastpage

263

Abstract

An integrated in situ analyzer for ATP concentration (IISA-ATP) has been developed in this study. Concept of IISA-ATP that has function for cell lysis, extracellular ATP elimination, ATP quantification with luciferin-luciferase reaction and in situ calibration is proposed in this study. A simplified prototype of PDMS based microfluidic chip that has a coiled microchannel pattern for optical detection is developed with micro fabrication technique. As a result of evaluation of the prototype, more than 10^-10 M range of ATP was successfully detected

Keywords

bioMEMS; biochemistry; biological techniques; bioluminescence; cellular biophysics; chemical analysis; chemical variables measurement; ecology; enzymes; microchannel flow; microorganisms; molecular biophysics; seawater; ATP concentration analysis; ATP quantification; IISA-ATP; cell lysis; coiled microchannel pattern; extracellular ATP elimination; in situ ATP quantitative analysis system; in situ calibration; luciferin-luciferase reaction; marine ecosystems; microbial cells; microfabrication technique; microfluidic chip; optical detection; polydimethylsiloxane; seawater samples; Biological materials; Calibration; Ecosystems; Extracellular; Microfluidics; Optical detectors; Optical materials; Pollution measurement; Prototypes; Sea measurements; ATP; IISA-ATP; Luciferin-Luciferase reaction; PDMS; in-situ analysis;

fLanguage

English

Publisher

ieee

Conference_Titel

Microtechnologies in Medicine and Biology, 2006 International Conference on

Conference_Location

Okinawa

Print_ISBN

1-4244-0338-3

Electronic_ISBN

1-4244-0338-3

Type

conf

DOI

10.1109/MMB.2006.251544

Filename

4281362