Fabrication of Microbioreactors with an Optimized Structure Designed for High Density Culture of Hepatocyte

Author

Provin, Christophe ; Takano, Kiyoshi ; Shirakashi, Ryo ; Sakai, Yasayuki ; Fujii, Teruo

Author_Institution

Inst. of Ind. Sci., Tokyo Univ.

fYear

2006

fDate

9-12 May 2006

Firstpage

267

Lastpage

270

Abstract

In order to perform cell culture from soft tissue in good condition, i.e. maintaining high viability and keeping specific physiological function, scaffolds with microstructured surface mimicking in vivo environment is necessary. In this paper we proposed PDMS microbioreactors with high surface/volume ratio for hepatocyte culture. These PDMS microbioreactors were designed for cells to attach in high density (over 10⁸ cells/cm³), and were characterized in terms of flow resistance, which is important for mass transport of nutrients and oxygen

Keywords

bioreactors; cellular biophysics; polymers; tissue engineering; PDMS microbioreactor fabrication; cell culture; flow resistance; hepatocyte culture; optimized structure design; scaffolds; soft tissue; tissue engineering; Biochemistry; Biodegradable materials; Biological materials; Bioreactors; Design optimization; Fabrication; Immune system; Microstructure; Surface resistance; Textile industry; 3D scaffold; cell culture; designed microstructure; tissue engineering;

fLanguage

English

Publisher

ieee

Conference_Titel

Microtechnologies in Medicine and Biology, 2006 International Conference on

Conference_Location

Okinawa

Print_ISBN

1-4244-0338-3

Electronic_ISBN

1-4244-0338-3

Type

conf

DOI

10.1109/MMB.2006.251546

Filename

4281364