Title :
The Varifocal Scanning Micro-Zooming Technique: A New Method for Measuring the Neurite Length in Three Dimensional Spaces
Author :
Zhang Wei ; Zheng Xianju ; Li Jiye ; Peng Jin
Author_Institution :
Comput. Eng. Dept., Chengdu Electromech. Coll., Chengdu, China
Abstract :
Neurite length measurement is very important in describing neuron´s physiological states and of morphological parameters. Combining computer technology and modern microscopic optical technology can develop a high precision measurement for measuring the neurite length in three-dimensional space. Through record Zeiss microscope spiral rotation angle in two different planes and Matlabs excellent image process compatibility, the neurite total length and the distance between two adjacent plane can be stored and analysised. Single and connect centerlines alone neurite are extracted. Based on the fast neurite centerlines extracting systems and automatic data processing, we have produced an automated neurite labeling and measuring system that could be used for quantitatively studying neuronal structure in 3D cultures.
Keywords :
biomedical measurement; biomedical optical imaging; length measurement; medical image processing; neurophysiology; optical microscopy; Matlab; Zeiss microscope; automated neurite labeling system; automated neurite measuring system; automatic data processing; image processing; neurite length measurement; neuron morphological parameters; neuron physiological states; neuronal structure; spiral rotation angle; three dimensional spaces; varifocal scanning micro-zooming technique; Computer languages; Data mining; Extraterrestrial measurements; Image analysis; Length measurement; Optical computing; Optical microscopy; Optical recording; Space technology; Spirals;
Conference_Titel :
Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th International Conference on
Conference_Location :
Chengdu
Print_ISBN :
978-1-4244-4712-1
Electronic_ISBN :
2151-7614
DOI :
10.1109/ICBBE.2010.5517791