Detection and quantitation of insulin-like growth factor-1 in velvet antler by an enzyme-linked immunosorbent assay

Author

Chunjuan, Liu ; Shouqin, Zhang ; Dongsheng, Li ; Jianpeng, Dou

Author_Institution

Coll. of Biol. & Agric. Eng., Jilin Univ., Changchun, China

fYear

2011

fDate

16-18 April 2011

Firstpage

1162

Lastpage

1168

Abstract

An enzyme-linked immunosorbent assay (ELISA) was used to detect the content of insulin-like growth factor-1 (IGF-1) in velvet antler water extract. The ELISA was validated with respect to parallelism, recovery, intra-assay variation, and inter-assay variation. The practical working range of detection was estimated to be 0.8 to 200 ng/mL, and the recovery was 96.7% or more. The effect of sample matrix was assessed by serial dilution. The result indicated that this ELISA method is a reliable, safe, and cost-effective tool for determination of IGF-1 content in velvet antler and serum.

Keywords

biotechnology; enzymes; medicine; ELISA method; IGF-1; enzyme-linked immunosorbent assay; insulin-like growth factor-1; interassay variation; intraassay variation; medicine; parallelism; recovery; serial dilution; serum; velvet antler water extract; Accuracy; Blood; Correlation; Educational institutions; Immune system; Interference; Plasmas; ELISA; IGF-1; plasma; velvet antler;

fLanguage

English

Publisher

ieee

Conference_Titel

Consumer Electronics, Communications and Networks (CECNet), 2011 International Conference on

Conference_Location

XianNing

Print_ISBN

978-1-61284-458-9

Type

conf

DOI

10.1109/CECNET.2011.5768437

Filename

5768437