Induction of Cytochrome CYP4F3 in Hydroquinone-Treated HL-60 Cells

Author

Li, Yuhong ; Wang, Hong ; Hu, Jing ; ZHu, Zhongling ; ZHao, Zhiwei ; Bi, Yongyi

Author_Institution

Sch. of Public Health, Wuhan Univ., Wuhan, China

fYear

2010

fDate

18-20 June 2010

Firstpage

1

Lastpage

5

Abstract

Cytochrome P450 4F3 (CYP4F3) catalyzes the inactivation of leukotriene B4 by ω-oxidation in human neutrophils. Here, we examined the mRNA and protein expression of the CYP4F3 in HL-60 cells, an acute promyelocytic leukemia cell line, in the presence of hydroquinone(HQ). We found hydroquinone led to a decrease in cell proliferation and a increase in cell apoptosis in a dose-dependent and time-dependent manner, it also most strongly induced the expression of the CYP4F3. CYP4F3 appears to be responsible for the cell proliferation and cell apoptosis, which may be play a role in the toxicity of hydroquinone.

Keywords

biochemistry; biological techniques; catalysis; cellular biophysics; electrophoresis; gels; molecular biophysics; proteins; ω-oxidation; P450 4F3 catalysis; cell apoptosis; cell proliferation; cytochrome CYP4F3; human neutrophils; hydroquinone-treated HL-60 cells; leukotriene B4; mRNA; promyelocytic leukemia cell line; protein expression; single-cell gel electrophoresis; toxicity; Cells (biology); DNA; Electrokinetics; Humans; Manufacturing; Oxidation; Proteins; RNA; Tail; USA Councils;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th International Conference on

Conference_Location

Chengdu

ISSN

2151-7614

Print_ISBN

978-1-4244-4712-1

Electronic_ISBN

2151-7614

Type

conf

DOI

10.1109/ICBBE.2010.5518232

Filename

5518232