Effect of light irradiation and immobilization of carbon nanotubes on kinetic parameters corresponding to Michaelis — Menten equation, for NADH sensors based on phenothiazine graphite modified electrodes

Author

Ungurecan, M.-L. ; Gligor, D.-M.

Author_Institution

Chem. Dept., Tech. Univ. of Cluj-Napoca, Cluj-Napoca, Romania

Volume

3

fYear

2010

fDate

28-30 May 2010

Firstpage

1

Lastpage

6

Abstract

The presence of light irradiation and immobilization of carbon nanotubes on graphite electrodes modified with polyphenothiazine formaldehyde (PPF) and bis-phenothiazin-3-yl methane (BPhM) leads to an increasing of sensitivity for NADH detection (calculated as I_max/K_M ratio). The paper presents the analogical modelling and numerical simulation for fitting the Michaelis-Menten equation to experimental data by a regression analysis. The program determines the theoretical kinetic parameters: K_M and I_max and they are compared with the values of the same parameters experimentally determined.

Keywords

amperometric sensors; biochemistry; biological techniques; carbon nanotubes; catalysis; electrodes; enzymes; graphite; molecular biophysics; nanosensors; nanotube devices; oxidation; photoelectrochemistry; radiation effects; reaction kinetics theory; reduction (chemical); regression analysis; C; Michaelis-Menten equation; NADH detection; NADH sensor; amperometric detection; analogical modelling; bis-phenothiazin-3-yl methane; carbon nanotube immobilization; coenzyme; electrocatalytic oxidation; kinetic parameters; light irradiation; nicotinamide adenine dinucleotide; numerical simulation; phenothiazine modified graphite electrodes; photoelectrocatalytic oxidation; polyphenothiazine formaldehyde; redox mediators; regression analysis; sensitivity; Carbon nanotubes; Chemical technology; Electrodes; Electrons; Equations; Kinetic theory; Numerical models; Numerical simulation; Oxidation; Polymer films; Michaelis-Menten equation; NADH; Phenothiazine derivatives; carbon nanotubes; modified graphite electrodes; numerical modelling; simulation;

fLanguage

English

Publisher

ieee

Conference_Titel

Automation Quality and Testing Robotics (AQTR), 2010 IEEE International Conference on

Conference_Location

Cluj-Napoca

Print_ISBN

978-1-4244-6724-2

Type

conf

DOI

10.1109/AQTR.2010.5520775

Filename

5520775