Characterization of a biomolecular fuel delivery device under load

We describe the design, implementation, and characterization of a biomolecular RNA fuel delivery device under load. The device is based on genelet technology, and is capable of driving dynamic behavior in complex nucleic acid circuits. Here we closely examine the behavior of a system consisting of the device driving the operation of a simple, fluorescently-labeled DNA probe. We use a simple model to characterize the stability region of the system and the effect of changing load conditions. Finally, we validate these analytical observations with basic experiments.