Micromachined cellular characterization system for studying the biomechanics of individual cells

Author

Ayliffe, H. Edward ; Rabbitt, Richard D. ; Tresco, Patrick A. ; Frazier, A. Bruno

Author_Institution

Dept. of Bioeng., Utah Univ., Salt Lake City, UT, USA

Volume

2

fYear

1997

fDate

16-19 Jun 1997

Firstpage

1307

Abstract

Micromachining technologies have enabled the development of microchannel devices for studying the migration behavior of individual living cells in stable chemotactant gradients. A micromachined three-dimensional system for cellular characterization was designed, fabricated, and tested. Human leukocytes were characterized as they crawled on multiple surfaces toward a distant chemotactic source. Excellent optical clarity was maintained by constructing the microchannels from polished glass and polyimide. Results of the system design and various environmental effects on cellular morphology and motility are presented

Keywords

biological techniques; biomechanics; blood; cell motility; cellular biophysics; micromachining; cellular migration behavior; cellular morphology; distant chemotactic source; environmental effects; human leukocytes; individual cells biomechanics study; microchannels; micromachined cellular characterization system; optical clarity; polished glass; polyimide; stable chemotactant gradients; Adhesives; Biomechanics; Biomedical optical imaging; Chemistry; Glass; Microchannel; Micromachining; Surface morphology; Surface treatment; White blood cells;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid State Sensors and Actuators, 1997. TRANSDUCERS '97 Chicago., 1997 International Conference on

Conference_Location

Chicago, IL

Print_ISBN

0-7803-3829-4

Type

conf

DOI

10.1109/SENSOR.1997.635476

Filename

635476