Stimulated emission depletion with sub-ns pulses

Author

Westphal, V. ; Blanca, C. ; Kastrup, L. ; Hell, S.W.

Author_Institution

Max-Planck-Inst. fur Biophys. Chem., Gottingen, Germany

fYear

2003

fDate

22-27 June 2003

Firstpage

717

Abstract

We have designed a new stimulated emission depletion microscope that applies longer STED pulses (>100 ps) than commonly used (5-10 ps). It is found that longer pulses have a positive effect on photostability of the fluorescent molecules. Utilizing ultrafast shutters, the recovery of the fluorescence signal after shutting the STED beam off was determined on timescales between 100 ns and 10 ms. We examined a number of dyes that could be used in biological applications, e.g. to measure changes in pH.

Keywords

biological techniques; dyes; fluorescence; high-speed optical techniques; molecular biophysics; optical design techniques; optical elements; optical microscopes; pH; 100 ns to 10 ms; biological application; dyes; fluorescent molecule; longer pulse; pH change measurement; photostability; stimulated emission depletion microscope design; timescales; ultrafast shutter; Biomedical optical imaging; Chemistry; Delay; Fluorescence; Optical diffraction; Optical microscopy; Optical pulses; Optical saturation; Stimulated emission; Ultrafast optics;

fLanguage

English

Publisher

ieee

Conference_Titel

Lasers and Electro-Optics Europe, 2003. CLEO/Europe. 2003 Conference on

Print_ISBN

0-7803-7734-6

Type

conf

DOI

10.1109/CLEOE.2003.1313786

Filename

1313786

Link To Document

https://search.isc.ac/dl/search/defaultta.aspx?DTC=49&DC=3182234