Title :
Stimulated emission depletion with sub-ns pulses
Author :
Westphal, V. ; Blanca, C. ; Kastrup, L. ; Hell, S.W.
Author_Institution :
Max-Planck-Inst. fur Biophys. Chem., Gottingen, Germany
Abstract :
We have designed a new stimulated emission depletion microscope that applies longer STED pulses (>100 ps) than commonly used (5-10 ps). It is found that longer pulses have a positive effect on photostability of the fluorescent molecules. Utilizing ultrafast shutters, the recovery of the fluorescence signal after shutting the STED beam off was determined on timescales between 100 ns and 10 ms. We examined a number of dyes that could be used in biological applications, e.g. to measure changes in pH.
Keywords :
biological techniques; dyes; fluorescence; high-speed optical techniques; molecular biophysics; optical design techniques; optical elements; optical microscopes; pH; 100 ns to 10 ms; biological application; dyes; fluorescent molecule; longer pulse; pH change measurement; photostability; stimulated emission depletion microscope design; timescales; ultrafast shutter; Biomedical optical imaging; Chemistry; Delay; Fluorescence; Optical diffraction; Optical microscopy; Optical pulses; Optical saturation; Stimulated emission; Ultrafast optics;
Conference_Titel :
Lasers and Electro-Optics Europe, 2003. CLEO/Europe. 2003 Conference on
Print_ISBN :
0-7803-7734-6
DOI :
10.1109/CLEOE.2003.1313786
