The accuracy and precision of signal source localization with tetrodes

Four-sensor microelectrodes, commonly referred to as tetrodes, have the ability to significantly increase the signal-to-noise ratio of neuronal extracellular recordings. They also provide spatio-temporal information about extracellular action potentials (EAP) which may be used to localize and resolve individual neuronal signal sources. Since the relative position of sensors and neurons whose EAPs are recorded is not known during in vivo experiments, the accuracy and precision of neuronal source localization algorithms remain untested. In this study, electrical signals generated by a stimulator were recorded simultaneously with four recording micropipettes immersed in artificial cerebrospinal fluid. The location of the source was estimated using the multiple signal classification algorithm, with an accuracy and precision of ~4 μm and ~7 μm, respectively. These results suggest that in vivo localization and resolution of individual neuronal sources is feasible.