A novel single compartment in vitro model: Perflurocarbons for electrophysiological studies of the rat urinary bladder

This study presents a novel single compartment model for in vitro electrophysiological studies of the rat urinary bladder. We tested the functionality and suitability of FC-770 (a Perflurocarbon) for in vitro recording of nerve activity arising from the bladder in a single compartment setup. We have also favorably tested stimulation of the bladder via the bladder nerves in FC-770. The organ viability was monitored by recording spontaneous contractions of the bladder for a certain time. We propose the use of FC-770 as a fluid for nerve recording/stimulation in vitro as well as for maintaining organ viability, over the commonly used two compartmental methods.