Reproducibility investigations and different experiments with a new type of electronically-conducting-polymer based bipotentiostatic uric acid sensor

Author

Sántha, Hunor

Author_Institution

Dept. of Electron. Tecnol., Budapest Univ. of Technol. & Econ., Hungary

fYear

2001

fDate

2001

Firstpage

268

Lastpage

272

Abstract

Reproducibility investigations and different experiments with a new type of electronically-conducting-polymer based bipotentiostatic uric acid sensor have been conducted. The sensor was constructed by using dodecyl sulfate doped poly-N-methyl-pyrrole for enzyme immobilization. This polymer film can incorporate the enzyme in one step during electropolymerisation. By the bipotentiostatic measuring method, the two working electrodes (with and without the enzyme) are analogously prepared and polarized and the currents in the two circuits are measured in parallel in time. The layout, which consists of 4 electrode surfaces (W1, W2, reference, counter) was prepared by thick film technology on sintered alumina ceramic substrates. The reproducibility of the characteristics using various electrode materials (Au, Pt), and the influence of the temperature or current density of the polymerisation have been investigated. Different pre-treatments of the working electrodes have been applied. Comparisons of two types of uricase enzymes have been made

Keywords

biosensors; conducting polymers; current density; electrochemical electrodes; electrochemical sensors; hybrid integrated circuits; polymer films; polymerisation; proteins; thick film circuits; Al₂O₃; Au; Au electrodes; Pt; Pt electrodes; bipotentiostatic measuring method; circuit currents; conducting-polymer based bipotentiostatic uric acid sensor; dodecyl sulfate doped poly-N-methyl-pyrrole; electrode materials; electrode surfaces; electronically-conducting-polymer based bipotentiostatic uric acid sensor; electropolymerisation; enzyme immobilization; enzyme incorporation; polarized electrodes; polymer film; polymerisation current density; polymerisation temperature; reproducibility; sintered alumina ceramic substrates; thick film technology; uricase enzymes; working electrode pre-treatment; working electrodes; Biochemistry; Ceramics; Counting circuits; Current measurement; Electrodes; Polarization; Polymer films; Reproducibility of results; Thick films; Time measurement;

fLanguage

English

Publisher

ieee

Conference_Titel

Electronics Technology: Concurrent Engineering in Electronic Packaging, 2001. 24th International Spring Seminar on

Conference_Location

Calimanesti-Caciulata

Print_ISBN

0-7803-7111-9

Type

conf

DOI

10.1109/ISSE.2001.931077

Filename

931077