A microfabricated device for the study of the sieving effect in protein electrophoresis

Author

Furlong, Cynthia D. ; Beebe, David J.

Author_Institution

Dept. of Biomed. Eng., Louisiana Tech. Univ., Ruston, LA, USA

Volume

1

fYear

1996

fDate

31 Oct-3 Nov 1996

Firstpage

248

Abstract

Many of the process factors involved in polyacrylamide gel electrophoresis of proteins are not well understood. A miniaturized device containing an “artificial gel” sieving matrix has been microfabricated. The well-defined structural features offer advantages of simplifying factors such as the pore size of the gel and the frictional forces that occur between the gel and the migrating molecule. These studies may lead to the ability to define and model optimal separation parameters for specific protein separations

Keywords

biological techniques; biotransport; electrophoresis; gels; micromachining; micromechanical devices; proteins; separation; anodic bonding; artificial gel sieving matrix; frictional forces; microfabricated device; micromachining; miniaturized device; optimal separation parameters; polyacrylamide gel electrophoresis; polysilicon; pore size; protein electrophoresis; sieving effect; specific protein separations; Biomedical engineering; Electrokinetics; Friction; Humans; Polymer gels; Protein engineering; Prototypes; Separation processes; Solvents; Visualization;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 1996. Bridging Disciplines for Biomedicine. Proceedings of the 18th Annual International Conference of the IEEE

Conference_Location

Amsterdam

Print_ISBN

0-7803-3811-1

Type

conf

DOI

10.1109/IEMBS.1996.656937

Filename

656937