A dynamic perfusion bioreactor approach for engineering respiratory tissues in-vitro

In vitro culture of respiratory tissues poses many challenges due to the intrinsic complexity of the respiratory system. Multiple cellular phenotypes comprise the respiratory epithelium and operate under dynamic, gas-interchanging conditions that should be replicated for near-physiologic cultivation of functional tissues in vitro. A novel biomimetic perfusion bioreactor system has been proposed to reconstitute key functional conditions of the human lung. This portable system consists of several biologically-inspired components: (i) a 3-dimensional (3-D) elastomeric soft tissue scaffold construct, (ii) a mechanical actuator, (iii) a perfusion system and (iv) gaseous exchange capabilities. These integrated components operate synergistically to create a unique, dynamic air-liquid interface (ALI) environment that allows controlled application of physiological and pathological strain while complementing standard cell culture techniques. This system holds potential for engineering 3-D tissues to meet growing demand for a range of applications, from more ethical and efficient pharmaceutical screening to clinical graft transplants.