Response surface approach for the Biodegradation of pretreated coir pith using Aspergillus nidulans SU04 for cellulase production

Author

Jabasingh, S.A. ; Nachiyar, C.V.

Author_Institution

Dept. of Chem. Eng., Sathyabama Univ., Chennai, India

fYear

2011

fDate

20-22 July 2011

Firstpage

711

Lastpage

716

Abstract

The present study is aimed at the synthesis of cellulase by Aspergillus nidulans SU04 using coir pith as chief substrate. The rate of enzymatic hydrolysis is increased by alkalipre-treatment with Sodium hydroxide. The effect of fermentation conditions including coir pith weight, moisture content, initial pH and growth temperature on cellulase activity and cellulase yield were investigated by response surface methodology (RSM) employing a four- factor- five- level central composite design (CCD). The results of Fourier transform infrared spectroscopy (FTIR), X-Ray diffraction (XRD) and Scanning electron microscopy (SEM) of coir pith showed structural changes through pretreatment, in favour of enzymatic hydrolysis.

Keywords

Fourier transform spectra; X-ray diffraction; biotechnology; environmental degradation; enzymes; infrared spectra; moisture; response surface methodology; scanning electron microscopy; sodium compounds; Aspergillus nidulans SU04; Fourier transform infrared spectroscopy; X-Ray diffraction; alkalipre-treatment; biodegradation; cellulase activity; cellulase production; cellulase synthesis; cellulase yield; central composite design; enzymatic hydrolysis; fermentation conditions; growth temperature; moisture content; pretreated coir pith; response surface approach; scanning electron microscopy; sodium hydroxide; Aspergillus nidulans SU04; CMCase; Cellulase yield; Pretreated coir pith; Response surface methodology; Solid state fermentation;

fLanguage

English

Publisher

iet

Conference_Titel

Sustainable Energy and Intelligent Systems (SEISCON 2011), International Conference on

Conference_Location

Chennai

Type

conf

DOI

10.1049/cp.2011.0455

Filename

6143404