The process of waste water with benzyls by comamonas testosteroni

Waste water with benzyls was common in chemical industry and it was quite poisonous to the environment and animals. Microbe was usually used to deal with chemical sewage. Universality to benzyls was an essential to microbe for process of this kind of sewage. Comamonas testosteroni was isolated by key laboratory of Tianjin Polytechnic University. It could grow well at about 37°C with the rotational speed of 200 rpm. It was found out to be powerful to biodegrade chemicals with benzyls using them as the only carbon source. Five substrates had been identified including diethyl terephthalate, dimethyl terephthalate, mono-methyl terephthalate, terephthalic acid and protocatechuic acid. Ultraviolet spectrophotometer and HPLC was used to monitor the degradation process and reasonable degradation pathway and the species of enzymes comamonas testosteroni excreted during degradation could be presumed. The degradation pathway might include the breaking of ester bond, the oxidation of hydroxyl, the decarboxylation, the breaking of benzyl and so on revealing that the enzymes might include hydrolase, oxidase, decarboxylase and so on. Benzyl containing chemicals were usually poisonous, so the various degradation capability of comamonas testosteroni ensure it a kind of suitable bacillus to clean the environment.