Fabrication and characterization of porous silicon nanoparticles for siRNA delivery

Author

Kopermsub, Phikunthiong ; Mayen, Varissaporn ; McInnes, Steven ; Voelcker, Nicolas H.

Author_Institution

Nat. Nanotechnol. Center, Nat. Sci. & Technol. Dev. Agency, Klong Luang, Thailand

fYear

2011

fDate

15-18 Aug. 2011

Firstpage

830

Lastpage

832

Abstract

RNA interference (RNAi) using small interfering RNA (siRNA) has been proposed for the therapy of human diseases and in particular cancers. Due to the instability and physicochemical properties of siRNA, the development of siRNA delivery system has attracted much attention. This study aimed at the fabrication of porous silicon nanoparticles (pSi NPs) as carriers for siRNA. pSi NPs possess a high surface area and are biodegradable and biocompatible. pSi NPs with two types of surface chemistry (oxidized and amine-functionalized) were fabricated and characterized. The surface area of pSi NPs was found to be 390 m²/g with a pore width of 9.2 nm. To investigate siRNA loading efficiency of pSi NPs, duplex siRNA was incubated with nanoparticles for 1 hour. The loading capacity of oxidized pSi NPs and amine-functionalized pSi NPs was found to be 1.95 μg/mg particles and 2.02 μg/mg particles, respectively. Further investigation on cellular uptake and gene silencing of siRNA-loaded pSi nanoparticles will be conducted to provide more details on their possible use for siRNA delivery.

Keywords

RNA; drug delivery systems; molecular biophysics; nanofabrication; nanomedicine; nanoparticles; porous materials; silicon; surface chemistry; RNA interference; RNAi; Si; amine functionalized silicon nanoparticle surface; cancer therapy; duplex siRNA; oxidized silicon nanoparticle surface; porous silicon nanoparticle characterization; porous silicon nanoparticle fabrication; siRNA carriers; siRNA delivery system; siRNA instability; siRNA loading efficiency; siRNA physicochemical properties; small interfering RNA; surface chemistry; Films; Loading; Nanoparticles; Scanning electron microscopy; Silicon; Surface morphology; amine-functionalization; porous silicon nanoparticles; siRNA delivery;

fLanguage

English

Publisher

ieee

Conference_Titel

Nanotechnology (IEEE-NANO), 2011 11th IEEE Conference on

Conference_Location

Portland, OR

ISSN

1944-9399

Print_ISBN

978-1-4577-1514-3

Electronic_ISBN

1944-9399

Type

conf

DOI

10.1109/NANO.2011.6144461

Filename

6144461