Title :
Statistical analysis of a HPLC method for microcystins determination in drinking water
Author :
Ning, Lu ; Jingxian, Sun ; Fanxin, Kong ; Zhang Dong
Author_Institution :
Shanghai Municipal Water Resource Dev. & Utilization Nat. Eng. Center Co., Ltd., Shanghai, China
Abstract :
Statistical analysis of high performance liquid chromatography (HPLC) method for the determination of 0.1~1.0 mg·L-1 microcystins (MCs) in drinking water was investigated to optimize the instrument operating conditions and reduce the detection limit. The detection limits were obtained by two ways. One through method detection limit (MDL) and the other through detection limit from the calibration curve (DTC). The Cochran test results revealed the homoscedasticity of the HPLC method. The effect of system drift on the calibration curve was noticed by the lack of fit (LOF) test. MDL yielded a less reliable detection limit than DTC. The detection limit of HPLC method for MC-LR was 0.009 mg·L-1 under recommended operating conditions.
Keywords :
chromatography; microorganisms; statistical testing; toxicology; water pollution; water quality; water supply; Cochran test; HPLC method; LOF test; calibration curve; drinking water; high performance liquid chromatography; homoscedasticity; lack of fit test; method detection limit; microcystins determination; statistical analysis; Algae; Calibration; Monitoring; Peptides; Risk management; Toxicology; Water resources; Cochran test; detection limit; lack of fit test; microcystins;
Conference_Titel :
Electric Technology and Civil Engineering (ICETCE), 2011 International Conference on
Conference_Location :
Lushan
Print_ISBN :
978-1-4577-0289-1
DOI :
10.1109/ICETCE.2011.5775187
