The experimental studies of bio-particle trapping using electrodeless dielectrophoresis

The ability to manipulate particles, especially living cells, is fundamental needed to many biological and medical applications, including isolation and detection of sparse cancer cells, concentration of cells from dilute suspensions, separation of cells according to specific properties, and trapping and positioning of individual cells for characterization. The electrodeless dielectrophoretic (EDEP) trapping is composed of conductless tetragon structures in micro-chip. We have improved the design of EDEP geometries, correlated particle acted by DEP effects with electrical-field distributions determined through insulating microstructures produce non-uniform electric fields to drive particles in microsystems. This study is attempt to research on microfluidic trapping by using DEP force and to investigate the relation on important parameters of microchip device for our design to comprehend the factors containing the spacing between two structures, the applied voltage and flow velocity.