Formulation of Low Intensity Direct Current Effects on Wound Healing of Skin using Genetic Programming

This study has two main purposes: Firstly, the effect of electrical stimulation was studied on experimental wound healing of skin. It investigates whether a triggering was possible on collagen synthesis in wound healing before the 3rd day of wounding, via measurements of hydroxyproline, a measure of collagen concentration in the wound tissue. Wound tissue hydroxyproline contents were determined with the effects of two different electric current intensities of 200 muA and 400 muA at 48 and 72 hours after wounding by Woessner´s Method. For both of the current intensities, statistically significant increases were found in hydroxyproline contents of current applied wounds with respect to their controls for 72 hours whereas decreased hydroxyproline content was found for 400 muA of current intensities at 48 hours. Secondly, it was aimed to model and formulate how electric current effects wound healing using Genetic Programming (GP) based on experimental results. The accuracy of the proposed GP formulation is defined quantiatively by standard deviation (SD=0.39) and correlation coefficient (R=0.85), which is found to be high.