Title :
Adherent cells localization based on Gaussian filtering in automated microinjection system
Author :
Ping Gao ; Haibo Huang ; Guodong Chen ; Liguo Chen ; Tao Chen
Author_Institution :
Jiangsu Key Lab. for Adv. Robot. Technol., Soochow Univ., Suzhou, China
Abstract :
This work is based on the cells in the microinjection system to process adherent cells image. Performing low-pass filtering to the Image of adherent cells, removing uneven illumination, segmenting foreground and background, removing invalid small areas, operating opening and closing operation, keeping the target area, calibrating centers of the cells in the original image.
Keywords :
adhesion; biomechanics; biomedical optical imaging; calibration; cellular biophysics; image segmentation; low-pass filters; medical image processing; Gaussian filtering; adherent cell image; adherent cell localization; automated microinjection system; calibrating centers; closing operation; low-pass filtering; operating opening; segmenting background; segmenting foreground; small area removal; Calibration; Cutoff frequency; Filtering; Image segmentation; Lighting; Microinjection; Microscopy; Removal of small areas; Segmentation; cellular localization; filtering;
Conference_Titel :
Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO), 2013 International Conference on
Conference_Location :
Suzhou
Print_ISBN :
978-1-4799-1210-0
DOI :
10.1109/3M-NANO.2013.6737406
