Coating method of micro pattern based on microscopic injection system

It´s of significance to study coating method of micro pattern, which plays an important role in the research of biological growth in cell scale. To avoid high cost for micro fabrication in traditional coating method and at the same time to improve flexibility, a novel coating approach based on microscopic injection system is proposed in the paper. In contrary to traditional micro injection, controlling pressure is just used to keep the balance of solution in injection pipette while interfacial force at tip between solution and substrate is used to drive out solution for coating. According to the proposed method, micro stripe and micro dot pattern coating are achieved in our experiment, which verifies the effectiveness of the method. Meanwhile, neural stem cells(NSCs) growth and distribution controlling results verifies the practical application value of the coating method.