A flow-through optical sensor system for label-free detection of proteins and DNA

Author

Petrou, Panagiota S. ; Zavali, Maria ; Raptis, Ioannis ; Kakabakos, Sotirios E. ; Misiakos, Konstantinos ; Beltsios, Konstantinos ; Ricklin, Daniel ; Lambris, John D.

Author_Institution

Immunoassay/Immunosensors Lab., Inst. of Radioisotopes & Radiodiagnostic Products, Greece

fYear

2009

fDate

25-28 Oct. 2009

Firstpage

27

Lastpage

30

Abstract

A regenerable flow-through sensor based on white light reflectance spectroscopy for label-free detection of proteins and DNA is presented. Detection is based on monitoring the interference wavelength shift caused by an increase in film thickness during bioreactions. By employing an anti-mouse IgG antibody/mouse IgG assay, a detection sensitivity of 150 pM was determined. In addition, the sensor response remained stable after up to 10 regeneration cycles. The sensor was applied for the quantitative determination of complement activation product C3b in human serum, a candidate biomarker for estimating the status of autoimmune diseases. Complement activation products in human serum could be detected at dilutions as high as 6000-fold. Detection of DNA hybridization at concentrations of 5 nM was easily achieved. The proposed sensor is characterized by low cost, short analysis time, high assay sensitivity and reliability.

Keywords

DNA; biosensors; light reflection; optical sensors; proteins; DNA hybridization; flow through optical sensor system; interference wavelength shift; label free detection; proteins; white light reflectance spectroscopy; Biosensors; DNA; Humans; Interference; Monitoring; Optical films; Optical sensors; Proteins; Reflectivity; Spectroscopy;

fLanguage

English

Publisher

ieee

Conference_Titel

Sensors, 2009 IEEE

Conference_Location

Christchurch

ISSN

1930-0395

Print_ISBN

978-1-4244-4548-6

Electronic_ISBN

1930-0395

Type

conf

DOI

10.1109/ICSENS.2009.5398129

Filename

5398129