Title :
A novel platform technology for the detection of genetic variations by surface plasmon resonance
Author :
Mertig, Michael ; Kick, Alfred ; Bonsch, Martin ; Katzschner, Beate ; Voigt, Jan ; Sonntag, Frank ; Schilling, Niels ; Klotzbach, Udo ; Danz, Norbert ; Begemann, Sebastian ; Herr, Alexander ; Jung, Martin
Author_Institution :
Max Bergmann Center of Biomater., Tech. Univ. Dresden, Dresden, Germany
Abstract :
We report on a novel approach to identify genetic variations based on the detection of specific polymerase-chain-reaction products by surface plasmon resonance. We use a recently developed, home-made spectrometer which exploits chips with integrated optics and microfluidics. The gold film at the chip surface is locally functionalized with single-stranded, thiol-modified probe DNA by applying a nanoliter dispenser. We discuss the chemical conditions for the achievement of maximum SPR signal strength and spot array density, and evaluate the SPR detection of selective binding of a set of 17 PCR products.
Keywords :
DNA; bioMEMS; biosensors; genetics; gold; infrared spectrometers; integrated optics; lab-on-a-chip; metallic thin films; micro-optomechanical devices; microsensors; molecular biophysics; optical sensors; surface plasmon resonance; Au; SPR detection; SPR signal strength; chemical conditions; genetic variations; gold film; home-made spectrometer; integrated optics; microfluidics; nanoliter dispenser; selective binding; single-stranded thiol-modified probe DNA; specific polymerase-chain- reaction products; spot array density; surface plasmon resonance; Genetics; Gold; Integrated optics; Microfluidics; Optical films; Plasmons; Polymers; Probes; Resonance; Spectroscopy;
Conference_Titel :
Sensors, 2009 IEEE
Conference_Location :
Christchurch
Print_ISBN :
978-1-4244-4548-6
Electronic_ISBN :
1930-0395
DOI :
10.1109/ICSENS.2009.5398247
