3D photo-crosslinking pattern scaffold for cell application by two-photon laser scanning system

Author

Wang, Yu-Fu ; Liang, Jen-I ; Lee, Chien-Te ; Yeh, Ming-Long ; Chen, Shean-Jen

Author_Institution

Inst. of Biomed. Eng., Nat. Cheng Kung Univ., Tainan, Taiwan

fYear

2011

fDate

20-23 Feb. 2011

Firstpage

491

Lastpage

494

Abstract

In this study, we fabricated a photo-crosslinking, biocompatible 3D Poly(ethylene) glycol (PEG) hydrogel scaffold with inner fibrin gel for cell adhesion. We tested the material cytocompatibility with 3T3 fibroblasts then processed the cell adhesion motif inside the scaffold at regular and L-shaped 3D structural pattern by two-photon laser scanning system for further cell manipulation and recording. The results show cell could survive under UV irradiation and grew in the inner fibrin gel scaffold. The desired pattern scaffold has potential to apply in cell guided migration and physiological signal recording.

Keywords

biomedical materials; hydrogels; laser applications in medicine; laser materials processing; two-photon processes; 3D photo-crosslinking pattern scaffold; 3T3 fibroblasts; PEG hydrogel scaffold; UV irradiation; biocompatible 3D Poly(ethylene) glycol; cell adhesion motif; cell application; cell guided migration; cell manipulation; inner fibrin gel; material cytocompatibility; physiological signal recording; two photon laser scanning system; Adhesives; Lasers; Materials; Optical device fabrication; Optical imaging; Photonics; Three dimensional displays; Hydrogel; Photon Cross-linking; Poly(ethylene) glycol (PEG); Two Photon Laser;

fLanguage

English

Publisher

ieee

Conference_Titel

Nano/Micro Engineered and Molecular Systems (NEMS), 2011 IEEE International Conference on

Conference_Location

Kaohsiung

Print_ISBN

978-1-61284-775-7

Type

conf

DOI

10.1109/NEMS.2011.6017400

Filename

6017400