A biosensor for detection of DNA sequences based on a 50MHz QCM electronic oscillator circuit

Author

Bustabad, E.A. ; García, G. ; Rodriguez-Pardo, L. ; Faria, J. ; Perrot, H. ; Gabrielli, C. ; Bucur, B. ; Lazerges, M. ; Rose, D. ; Compère, C. ; Arnau, A.

Author_Institution

Dipt. Tecnol. Electron., Univ. de Vigo, Vigo, Spain

fYear

2009

fDate

25-28 Oct. 2009

Firstpage

687

Lastpage

690

Abstract

This work deals with the development of a high sensibility DNA biosensor based on a 50MHz Quartz Crystal Microbalance (QCM) Oscillator Electronic Circuit. The designed QCM oscillator sensor is able to detect the presence of complementary DNAs in a solution that match the sequence on a given strand in function of the changes in the output frequency of the oscillator. The design is adapted so that the Barkhausen condition is satisfied even when the quartz is immerged in liquid media. Also a comparative study of the developed 50MHz biosensor in front of a QCM oscillator with smaller frequency is carried out, with object of checking if the sensibility of the oscillator increases, allowing to detect smaller concentrations of the complementary DNA.

Keywords

DNA; biosensors; circuit oscillations; crystal oscillators; microbalances; molecular biophysics; Barkhausen condition; DNA sequence detection; QCM electronic oscillator circuit; QCM oscillator sensor; biosensor; complementary DNA; frequency 50 MHz; oscillator output frequency; quartz crystal microbalance; Biosensors; Circuits; Crystalline materials; DNA; Electrodes; Frequency; Object detection; Oscillators; Q factor; Sequences;

fLanguage

English

Publisher

ieee

Conference_Titel

Sensors, 2009 IEEE

Conference_Location

Christchurch

ISSN

1930-0395

Print_ISBN

978-1-4244-4548-6

Electronic_ISBN

1930-0395

Type

conf

DOI

10.1109/ICSENS.2009.5398346

Filename

5398346