Title :
A portable and high efficiency system for cell electroporation under low voltage
Author :
Wu, Mengxi ; Wei, Zewen ; Zhao, Deyao ; Li, Zhihong
Author_Institution :
Sch. of Comput. & Inf. Eng., Peking Univ., Shenzhen, China
Abstract :
Based on a novel micro-fabricated electroporation (EP) chip, a portable EP system was designed and manufactured. The whole system consisted of a programmable and adjustable mini impulsator and micro EP chips. Large statistical data of transfection efficiency were determined under various pulse amplitudes, durations and intervals in this system for HEK-293 cells. High transfeciton efficiency (80%) was achieved under low voltage (~20V) in this system. A man-machine interface was developed by keyboard and LCD screen in the mini impulsator using single chip micyoco. The impulsator can be adjusted to accommodate its pulse electrical parameters to a variety of conditions. The system has been fabricated and is in probation. With further EP experiments on other kinds of cells, more guidance will be given for researchers to shorten EP time and enhance efficiency.
Keywords :
biocomputing; bioelectric phenomena; human computer interaction; keyboards; liquid crystal displays; microfabrication; portable instruments; HEK-293 cells; LCD screen; adjustable impulsator; cell electroporation; keyboard; man-machine interface; microfabricated electroporation chip; portable EP system; programmable impulsator; pulse amplitudes; pulse electrical parameters; transfeciton efficiency; Educational institutions; Electric fields; Electrodes; Keyboards; Low voltage; Micromechanical devices; User interfaces; HEK-293 cell; electroporation; low voltage transfer; man-machine interface; single chip micyoco system;
Conference_Titel :
Nano/Micro Engineered and Molecular Systems (NEMS), 2011 IEEE International Conference on
Conference_Location :
Kaohsiung
Print_ISBN :
978-1-61284-775-7
DOI :
10.1109/NEMS.2011.6017467
