Direct fabrication of single nanopore in glass capillary

Nanopores are increasingly utilized as tools for single molecule detection in biotechnology. We report a novel method of fabricating a nanopore by thermally pulling a glass micropipette sealed with the paraffin to form a nanocavity enclosed in the terminal and then rubbing the terminal to make the cavity throughout. The principle is based on the physical footprint of the paraffin gaseous substances inducing the formation of glass-coated during the glass pipet melted pulling. Using this method, the sandglass-shaped hollow nanostructure is created, with about 40 nm terminal diameter. The nanocavity was shown to have a pore size on the scale of a single molecule, through which we detected the IgA (30 nm diameter) molecules translocation.