Title :
Selected segmentation methods applied for DNA image cytometry
Author :
Goclawski, Jaroslaw ; Aniol, Patryk
Author_Institution :
Comput. Eng. Dept., Lodz Tech. Universitv
Abstract :
This paper presents new algorithm of plant nuclei segmentation for the purpose of DNA contents measurements. This algorithm is based on preliminary discrimination of nuclei objects in RGB colour space and then on local applying of some global thresholding methods in a rectangular ROI area. Segmented nuclei objects are zoomed more than in typical applications to improve quantitative analysis
Keywords :
DNA; image segmentation; molecular biophysics; DNA contents measurements; DNA image cytometry; RGB colour space; global thresholding methods; image segmentation; plant nuclei segmentation; quantitative analysis; segmented nuclei objects; selected segmentation methods; Cameras; DNA; Hardware; Histograms; Image segmentation; Integrated optics; Microscopy; Nuclear measurements; Optical distortion; Optical filters; Image segmentation; adaptive thresholding; integrated optical density; intensity histogram; local thresholding; maximum entropy; moment preserving thresholding; watershed segmentation;
Conference_Titel :
Perspective Technologies and Methods in MEMS Design, 2006. MEMSTECH 2006. Proceedings of the 2nd International Conference on
Conference_Location :
Lviv
Print_ISBN :
966-553-517-X
DOI :
10.1109/MEMSTECH.2006.288680
