Title :
Reconstruction problems in 3D for viral cryo electron microscopy
Author :
Gao, Wen ; Doerschuk, Peter C.
Author_Institution :
Sch. of Electr. & Comput. Eng., Purdue Univ., West Lafayette, IN, USA
Abstract :
Cryo electron microscopy of viruses provides 2D projections of the scattering intensity of the viral particle but the orientation of the projections is not known. We describe an approach to reconstructing the 3D scattering intensity in spite of the unknown projection orientations using nonlinear least squares ideas where the reconstruction is guaranteed to have the icosahedral symmetry known to be present in the viral particle
Keywords :
biological techniques; biology computing; cellular biophysics; electron microscopy; image reconstruction; least squares approximations; 3D; 3D scattering intensity; icosahedral symmetry; nonlinear least squares; orientation; reconstruction; scattering intensity; viral cryo electron microscopy; Computer viruses; Electron beams; Electron microscopy; Fourier transforms; Least squares methods; Particle scattering; Proteins; Signal reconstruction; Tires; Viruses (medical);
Conference_Titel :
Image Processing, 1998. ICIP 98. Proceedings. 1998 International Conference on
Conference_Location :
Chicago, IL
Print_ISBN :
0-8186-8821-1
DOI :
10.1109/ICIP.1998.723625
