Title :
Analysis of Relationship between Apoptosis and Changes of Ca2+ in HepG-2 induced by CSA with laser scanning confocal technology
Author :
Yu-bin Ji ; Lei Yu
Author_Institution :
Center of Res. & Dev. on Life Sci. & Environ. Sci., Harbin
Abstract :
Laser scanning confoncal technology was used to study relationship between apoptosis and changes of Ca2+ induced by CSA (Capparis spinosa L. total alkaloid, CSA) on human heptocarcinoma cell HepG-2. Killing effects of CSA on human heptocarcinoma cell HepG-2 was measured by MTT method, while morphological observation on HepG-2 cells was completed by fluorescence microscope. Apoptosis of HepG-2 cells induced by CSA was measured by flow cytometry. In addition, change of intracellular Ca2+ level of CSA on HepG-2 cells was observed by laser scanning confocal microscope. Results: CSA had obvious cytotoxicity on HepG-2 in a dose-dependent manner, and its IC50 was 162.4 mug/ml. CSA could induce characteristic apoptosic morphology of HepG-2 cells, and apoptosis percentage was significantly higher than control one. Migration of cells cycle from S phase to G2 phase had been blocked by CSA. Concentration of Ca2+ in HepG-2 had been increased by CSA, which was positive correlation with drug dosage. CSA had obvious effects of killing and inducing apoptosis on human heptocarcinoma cell HepG-2, and overload of Ca2+ might be involved in the se events.
Keywords :
biomedical optical imaging; biomembrane transport; calcium; cancer; cellular biophysics; drugs; optical microscopy; positive ions; CSA; Capparis spinosa L. total alkaloid; HepG-2; MTT method; apoptosis; calcium ions; cytotoxicity; drug dosage; flow cytometry; human heptocarcinoma cell; laser scanning confocal technology; Business; Cells (biology); Chemicals; Drugs; Fluorescence; Humans; Laser transitions; Microscopy; Probes; Research and development;
Conference_Titel :
Complex Medical Engineering, 2009. CME. ICME International Conference on
Conference_Location :
Tempe, AZ
Print_ISBN :
978-1-4244-3315-5
Electronic_ISBN :
978-1-4244-3316-2
DOI :
10.1109/ICCME.2009.4906581