Title :
Effect of qinglongyi polysaccharide on cation concentration in erythrocyte of S180 mice
Author :
Zou Xiang ; Ji Yu bin ; Ji Chen Feng ; Xiao Feng
Author_Institution :
Minist. of Educ., Eng. Res. Center of Natural Anticancer Drugs, Harbin
Abstract :
To study the effect of qinglongyi polysaccharide on cation concentration in erythrocyte of S180 mice a tumor mouse model was developed, and the mice were treated with high, medium, and low doses of qinglongyi polysaccharide through abdominal injection for 7 d. Erythrocytes were collected and erythrocyte suspensions were prepared. Change of Ca2+ concentration in the erythrocytes of S180 mice was measured by stained with Fluo-3/AM and observed with laser scanning confocal microscope, Change of H+ concentrations measured by stained with BCECF-AM and observed with laser scanning confocal microscope. Results showed Qinglongyi polysaccharide lowered the concentration of Ca2+ and H+ in erythrocytes of S180 mice, which is remarkable compared with negative control group(PLt0.05). Conclusion Qinglongyi polysaccharide can maintain the stability and balance of cation concentration in erythrocyte of S180 mice.
Keywords :
biochemistry; biological techniques; calcium; cellular biophysics; hydrogen ions; positive ions; sugar; BCECF-AM staining; Ca2+; Ca2+ concentration change; Fluo-3-AM staining; H+; H+ concentration change; Qinglongyi polysaccharide effects; S180 mice erythrocyte; cation concentration; erythrocyte suspensions; laser scanning confocal microscopy; tumor mouse model; Abdomen; Animals; Business; Drugs; Electronic mail; Laser transitions; Mice; Neoplasms; Probes; Suspensions;
Conference_Titel :
Complex Medical Engineering, 2009. CME. ICME International Conference on
Conference_Location :
Tempe, AZ
Print_ISBN :
978-1-4244-3315-5
Electronic_ISBN :
978-1-4244-3316-2
DOI :
10.1109/ICCME.2009.4906592
