Nanoengineered uric acid biosensor based on chemiluminescence

Author

Chaudhari, R.D. ; Joshi, A.B. ; Srivastava, Rajesh

Author_Institution

Dept. of Biosci. & Bioeng., IIT Bombay, Mumbai, India

fYear

2011

fDate

1-3 April 2011

Firstpage

1

Lastpage

2

Abstract

Uric acid (UA) is a nitrogenous end product of the purine catabolism in humans. Excessive production of UA may lead to gout, hyperuricemia and kidney disorders. The research here aims at developing an indirect enzyme based UA biosensor where UA is sensed from the amount of hydrogen peroxide (H₂O₂) produced. Pentacene loaded nanoparticles encapsulated in uricase loaded alginate microspheres (nano-in-micro) particles were synthesized. All formulations were characterized using DLS and imaged using Optical, SEM, TEM, CLSM and FTIR. These nano-in-micro particles were used for H₂O₂ and UA sensing. UA sensing with nano-in-micro system showed regression constant 0.977 in range of 0-0.1mg/ml. Thus chemiluminescent nano-in-micro particles can act as diagnostic markers for detection of UA.

Keywords

biosensors; chemiluminescence; enzymes; hydrogen compounds; molecular biophysics; nanomedicine; scanning electron microscopy; transmission electron microscopy; FTIR; H₂O₂; SEM; TEM; chemiluminescence; chemiluminescent microparticle; chemiluminescent nanoparticle; diagnostic markers; enzyme based UA biosensor; hydrogen peroxide; nanoengineered uric acid biosensor; nanoparticle; pentacene loaded nanoparticles; uricase loaded alginate microspheres; Biochemistry; Biosensors; Fluorescence; Nanoparticles; Pentacene; Suspensions; Uric acid; alginate; biosensor; pentacene;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioengineering Conference (NEBEC), 2011 IEEE 37th Annual Northeast

Conference_Location

Troy, NY

ISSN

2160-7001

Print_ISBN

978-1-61284-827-3

Type

conf

DOI

10.1109/NEBC.2011.5778591

Filename

5778591